Activation of small Rho GTPases by blebbistatin in PC12 cells / 동물의과학연구지
Journal of Biomedical Research
;
: 60-64, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-38428
ABSTRACT
Neuronal differentiation is a complex biological process accompanying cytoskeletal reorganization, including neurite outgrowth and growth cone formation. Therefore, neuronal differentiation is critically regulated by actin-related signaling proteins, such as small Rho GTPases, guanine nucleotide exchange factors (GEFs), and myosins. This study will demonstrate the change in activity of three small Rho GTPases, Rac, Cdc42, and Rho A, by treatment with blebbistatin (BBS), a specific inhibitor for myosin, during bFGF-induced neurite outgrowth in PC12 cells. Treatment with BBS induced morphological changes in growth cones and neurites during differentiation. A marked increase in protrusion and filopodia structures in growth cones, the shaft of neuritis, and cell membranes was observed in the cells treated with BBS. Activity of Rho GTPases showed the alterations in response to BBS. Activities of both Rac and Rho A were inhibited by BBS in a time-dependent manner. By contrast, Cdc42 activity was not changed by BBS. These results suggest that inactivation of myosin II by BBS induced morphological changes in neurites and growth cones and distinct regulation of three Rho GTPases during differentiation of PC12 cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pseudópodes
/
Fenômenos Biológicos
/
Proteínas
/
Membrana Celular
/
Miosinas
/
Células PC12
/
Neuritos
/
Cones de Crescimento
/
Proteínas rho de Ligação ao GTP
/
Fatores de Troca do Nucleotídeo Guanina
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of Biomedical Research
Ano de publicação:
2013
Tipo de documento:
Artigo
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