Study of total flavonoid in Ligustrum Lucidum Ait on lipometabolism regulation of HepG2 cell / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 205-206, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-389958
ABSTRACT
Objective To study molecular target of total flavonoid in Ligustrum Lucidum Ait(TFL) on lipometabolism regulation of HepG2 cell.Methods Treatment doses of total flavonoid in Ligustrum Lucidum Ait were determined by MTT and LDH.The expression of lipoprotein lipase(LPL),3-hydroxy-3-methylglutary-coenzyme A redutasein(HMGCR)and peroxisome proliferator-actived receptorsα(PPARα)mRNA of HepG2 cell were determined by reversed transcription polymerase chain reaction(RT-PCR).Results Increased expression of LPL mRNA and PPARαmRNA with TFL(10-6~10-4g/ml)were found in HepG2 cells after treated with TFL(10-5g/ml),but decreased expression of HMGCR mRNA was found after treated with TFL(10-5,10-4 g/ml).Conclusion The potential mechanisms of total flavonoid in Ligustrum Lucidum Ait's hypolipidmic effects may be with its functions of regulating lipoprotein degradation through PPARα-LPL pathway,or regulating steroid biosynthesis by reducing HMGCR.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
International Journal of Traditional Chinese Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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