Activin A Stimulates Mouse APCs to Express BAFF via ALK4-Smad3 Pathway
Immune Network
; : 196-202, 2011.
Article
em En
| WPRIM
| ID: wpr-39109
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and dendritic cells, and stimulates B cell proliferation, differentiation, survival, and Ig production. In the present study, we explored the effect of activin A on BAFF expression by APCs. METHODS: To investigate the effect of activin A on BAFF expression by mouse APCs, we measured the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA. RESULTS: Activin A markedly enhanced BAFF expression in mouse macrophages and dendritic cells at both the transcriptional and protein levels. SB431542, an activin receptor-like kinase 4 (ALK4) inhibitor, completely abrogated activin A-induced BAFF transcription. Furthermore, overexpression of DN-Smad3 abolished activin-induced BAFF expression at the transcriptional and protein levels. CONCLUSION: These results demonstrate that activin A can enhance BAFF expression through ALK4-Smad3 pathway.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Benzamidas
/
Células Dendríticas
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Ativinas
/
Receptores de Ativinas
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Proliferação de Células
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Dioxóis
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Macrófagos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2011
Tipo de documento:
Article