Cerebral ischemia induces astrocytes to express granulocyte colony stimulating factor and glial cell fine-derived neurotrophic factor / 中华神经科杂志

ABSTRACT

Objective To explore the neuroprotective mechanisms of granulocyte colony stimulating factor (G-CSF) in ischemic brain injury. Methods Brain tissues were taken out from MCAO and sham operated Sprague-Dawley rats 7 days after operation. The expression of G-CSFR, GDNF, MAP2 and GFAP was measured by using immunofluorescence co-staining. Results The expression of G-CSFR and GDNF were widely distributed in the neurons in normal brain tissues, not in the astrocytes. However, in ischemic peripheral zone, part of G-CSFR and GDNF positive cells merged with GFAP positive cells, in normal brain tissues, most G-CSFR positive cells were co-expressed with GDNF. Conclusion Cerebral ischemia induces astrocytes to express G-CSFR and GDNF, suggesting that endogenous neuroprotection by cerebral ischemia may be related with the expression and production of G-CSFR and GDNF in astrocytes in ischemic peripheral zone.