Expression of monocyte chemoattractant protein-1 in the interstitial cystitis patients / 中华泌尿外科杂志

ABSTRACT

Objective To investigate the levels of monocyte chemoattractant protein-1(MCP-1)in the bladder tissue and urine of female interstitial cystitis(IC)patients. Methods Thirty-five IC patients according tO the NIDDK IC diagnosis standard,20 urinary infection (UI) patients and 25 asymptomatic controls were collected.All IC patients were accepted 24 hour voiding diaries,O'LearySant IC Questionnaires,potassium sensitivity test (PST) and cystoscopy under anesthetic.RT-PCR and ELISA analyses were used to determine the levels of MCP-1 in the bladder tissue and urine specimens from women with IC、UI and controls.Immunohistochemistry staining was used to observe the distribution of MCP-1 in bladder tissue of IC. Results Urine MCP-1 was (74.1±36.9)pg/ml in IC patients,(280.65±68.9)pg/ml in UI patients and(10.8±6.9)pg/ml in asymptomatic controls(P＜0.01).Tissue MCP-1 was 76.2±24.0 in IC patients,99.5±30.1 in UI patients and 36.1±14.1 in asymptomatic controls(P＜0.01)by RT-PCR analyses.The MCP-1 degree increased in IC patients was between UI patients and asyrnptomatie controls.The severity of IC clinical symptom Was correlated with MCP1 levels. Conclusions The changes in the levels of MCP-1 are associated with IC.After excluding urinary infection,it would be useful for early diagnosis of IC by increased level of MCP-1.