Analysis of the transcription profiles of mouse thymus irradiated by γ-rays / 中华放射医学与防护杂志

ABSTRACT

Objective To investigate the dynamic changes of transcription profiles of mouse thymus gene expression in different times after 6 Gy γ-irradiation.Methods High-flux cDNA microarray technique was used irradiation,the numbers and types of differentially expressed genes were gradually decreased,for instance,the induced differential expression genes were involved in cell cycle,immunity and stress,apeptosis,signal transduction,transcription regulation,DNA synthesis and recombination,cystoskeleton,ion channel and transportation,metabolism,protein translation and synthesis,development and cell differentiation,etc.correlated cell cycle(3 up-regulatingCyclin G,Anxal,Fgf1 and 2 down-regulatingCdc2a,Cdc25b),5 genes correlated immune stress(4 up-regulatingIL-18,Casp1,IL-15,IL-7 and 1 down-regulatingCd28),7 genes correlated apoptosis(4 up-regulatingCaspl,Anxal,Perp,IL-7 and 3 down-regulatingPten,Api5 and Fas).Conclusions After 6 Gy irradiation,differentially expressed genes in mouse thymus is not only involved in many targets,levels and pathways,but also displayed an obvious difference in times.This reveals the regular pattern of differential expression genes in the process of injury and reconstitution in moderate dose irradiated mouse thymus.