Total antioxidants capacity in human plasma: A measurement method and some related experiments

ABSTRACT

Free radical and antioxidant are very interesting field of research in other country as in Viet Nam. They are mentioned as a new pathogennic agent. Objectives: Determination of total antioxidant capacity in plasma. Methods: Lag-time assay was established to measure Total Antioxidants Capacity (TAC) in human plasma of healthy volunteers (6 males, 4 females, aged 29,8+-5,2). Results: - TAC was 513.3+/-79.64 mM Trolox equivalents. TAC was significantly higher in men (568.83+/-41.08 mM Trolox equivalents) in comparison with women (430.00+/-28.15 mM Trolox equivalents). A main reason was a higher concentration of uric acid in men compared with women (397.5+-79.86 mM versus 245+-33.16 mM). Conclusions: TAC in plasma after drinking vegetable juice for 1 week (547.8+-80.67 mM Trolox equivalents) was significantly higher in comparison with before (513.3+-79.64 mM Trolox equivalents).