The value of plasma 8-iso-prostaglandin F2 alpha determination in the neonatal hyperunconjugated bilirubinemia / 中国医师进修杂志

ABSTRACT

Objective To investigate the changes of plasma 8-iso-prostaglandin F2 alpha (8-iso-PGF2α) before and after treatment in the neonatal hyper-uneonjugated bilirubinemia, so as to confirm theperoxidation damage of serum tolal bilirubin (TBil). Methods One hundred and twenty-six neonates who were 2-7 days old were divided into three groups according to TBil concentration group hyper-bihrubinemia A (HBE-A)(51 cases), group hyperbilirubinemia B (HBE-B)(25 cases), and healthy control group (50 cases). The serum TBil and unconjugated bilirubin contents were measured by automatic biochemical analyzer. The level of 8-iso-PGF2α in plasma was measured by enzyme linked immunosorbent assay. Results Plasma 8-iso-PGF2α level in healthy control group was (13.84±6.13)ng/L, 95% confidence interval was 10.88-18.02 ng/L.Plasma 8-iso-PGF2α level before treatment and 7 days after serum TBil recovering to be normal in group HBE-A and group HBE-B were (19.56±2.74), (14.13±5.93) ng/L and (22.30±6.33), (14.88±6.44) ng/L, respectively. Plasma 8-iso-PGF2α level increased with serum TBil increased. Plasma 8-iso-PGF2α level decreased with serum TBil decreased. Conclusions The neonatal hyper-unconjugated bilirubinemia can induce peroxidation damage.The determination of plasma 8-iso-PGF2α level may accurately evaluate the bilirubin- induced peroxidation in early stage and progression of jaundice, suggesting the antioxidants should be added to treat neonatal hyper-unconjugated bilirubinemia.