Association of gene polymorphism in promoter region of adiponectin gene and carotid artery intima-media thickness in patients with type 2 diabetes mellitus of Fujian / 中华内分泌代谢杂志

ABSTRACT

Objective To explore the association of-11377 site single nucleotide polymorphism in promoter region of adiponectin gene and carotid intima-media thickness(CIMT)in patients with type 2 diabetes mellitus.Methods The adiponectin gene-11377C→G polymorphism was identified by PCR-restriction fragment length polymorphism(RFLP)in 504 patients with type 2 diabetes mellitus(250 patients with increased CIMT and254 Datients with normal CIMT). Serum lipid and fasting plasma glucose were detected by full automatic biochemical analysor.fasting serum insulin(FINS) was measured by radioimmunoassay,and serum adiponectin level was assessed by ELISA.Results The frequencies of adiponectin gene-11377C→G genotype and allele were different between type 2 diabetic patients with normal and increased CIMT(P<O.01).In type 2 diabetic patients, the values of CIMT,diastolic blood pressure,low-density lipoprotein cholesterol (LDL-C)and homeostasis model assessment of insulin resistance (HOMA-IR) in-11377CC group were significantly lower than those in-11377CG +GG group(P<0.05 or P<O.01),but serum adiponectin level was higher in-11377CC group than that in -11377CG+GG group [(5.70 ±3.58 vs 4.72±2.34)mr/L,P<O.01].Variation at adiponectin gene-11377C→G was associated with increased CIMT in type 2 diabetic patients independent of serum adiponectin level(P= 0.037).Conclusion In type 2 diabetic patients of Fujian,the-11377C→G polymorphism of the adiponectin gene is associated with CIMT.