Calbindin-D28K Prevents Staurosporin-induced Bax Cleavage and Membrane Permeabilization
Experimental Neurobiology
;
: 173-177, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-39647
ABSTRACT
Calbindin-D28K has been implicated in the regulation of neuronal cell death. Previously, we demonstrated that calbindin-D28K prevents staurosporine (STS)-induced caspase activation and subsequent apoptosis in a neuronal cell line. However, the role of calbindin-D28K in STS-induced activation of calpain and necrotic cell death was not identified. Staurosporine induced the elevation of intracellular calcium after 1 hr of treatment. Overexpression of calbindin-D28K and presence of a calcium chelator, BAPTA, prevented the increase of calcium in STS-treated cells. Cleavage of Bax by calpain was prevented by the overexpressed calbindin-D28K. Permeabilization of the plasma membrane, a factor in necrosis, as well as apoptotic change of the nucleolus induced by STS, was prevented by calbindin-D28K. Thus, our study suggests that calbindin-D28K may exert its protective functions by preventing calpain activation in necrotic cell death, in addition to its effect on the caspase-apoptosis pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Calpaína
/
Linhagem Celular
/
Membrana Celular
/
Cálcio
/
Morte Celular
/
Apoptose
/
Estaurosporina
/
Calbindina 1
/
Membranas
/
Necrose
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Experimental Neurobiology
Ano de publicação:
2014
Tipo de documento:
Artigo
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