protective effect of Enalapril and weight reduction on podocyte lesions of obesity-related gLomerulop-athy rats / 中国医师杂志

ABSTRACT

Objective To investigate the expression of WT1 in pedecyte of obesity- related glomerulopathy (ORG) rats and the mechanism of enalapril and weight reduction-induced renal protection. Methods After the ORG model was established by high lipid forage for 24 weeks, the rata were divided into control rats (group A), ORG rats (group B), enalapril-treated ORG rats (group C), ORG rats with weight reduction (group D) and enalapril -treated ORG rats with weight reduction (group E). At the 8th week, 24 hour urine albumen (24hUA1b) was assayed, and the pathological changes of kidney were detected by optics microscopy and transmission electronic microscopy. Immunohistochemistry was used to detect the expression of WT1, a marker of pedocyte injury. Results In ORG rats, pedocyte number and density were declined remarkably compared with group A (respectively, P<0.01), and 24 hour UA1b and obvious kidney damage were sig- nificantly higher than those of group A (respectively, P<0.01 ). After treatment, pedocyte number and density in group (C, D, E) were significantly increased compared with group B (respectively, P<0.05, P<0.01). Meanwhile, 24honr UA1b was reduced and kidney dam- age was alleviated ( respectively, P <0. 01 ), and therapeutic alliance effect in group E outweighed than that in group C and D. Conclusion Changes of pedocyte in ORG correlated with degree of proteinuria and renal function impairment. Enalapril and weight reduction can re- strain the expression of WT1 in ORG, which can reduce proteinuria and lessen kidney damage.