Effects of Mumps Virus Nucleocapsid Protein on the Viral Replication and Apoptosis in VeroE6 Cells
Journal of Bacteriology and Virology
;
: 193-201, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-39998
ABSTRACT
Apoptosis, as a part of the natural defense mechanisms that protect against viral infection, plays a vital role in the pathogenic mechanisms. It also plays a key role in the pathogenesis of diseases including many viral diseases. Mechanisms of virus-induced apoptosis are not completely understood because of the complexity of the underlying biochemical cascades and all of the participating host factors. Mumps virus belongs to the genus Rubulavirus in the family Paramyxoviridae. It contains single stranded RNA genome with negative polarity. It was observed that mumps virus induced apoptosis in VeroE6 cells, and adsorption and penetration of mumps virus to cell membrane alone were not sufficient for the induction of cell death. When mumps virus was superinfected onto nucleocapsid protein (NP) expressing VeroE6 cells, cell viability and facterial titer were maintained until 13 and 12 day, respectively. The levels of p53, Bax, and Bcl-2 were increased in NP-expressing VeroE6 cells, and the increase in Bax, and Bcl-2 was outstanding. It was observed that NP protein did not directly affect the efficiency of the infection of mumps virus in NP-expressing VeroE6 cells. The levels of p53, and Bax were decreased in both mock-infected VeroE6 cells and NP-expressing VeroE6 cells infected with mumps virus. However, the Bcl-2 level was little affected by the virus infection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Paramyxoviridae
/
Viroses
/
RNA
/
Membrana Celular
/
Sobrevivência Celular
/
Genoma
/
Morte Celular
/
Apoptose
/
Rubulavirus
/
Nucleocapsídeo
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2003
Tipo de documento:
Artigo
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