Effect of FK506 on cell-surface antigen expression in porcine thymocytes / 中华器官移植杂志
Chinese Journal of Organ Transplantation
; (12): 164-165, 2000.
Article
em Zh
| WPRIM
| ID: wpr-400797
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WPRO
ABSTRACT
Objective To investigate the effect of FK506 on cell-surface antigen expression in porcine thymocytes.Methods After FK506 administration at a dose of 1 mg./kg body weight intramuscularly for 21 days the pigs were sacrificed and the thymus were removed.Cells isolated from the thymus were stained for two-color flow cytometric analysis in two-steps:
(1)incubation with a combination of monoclonal antibodies,and(2)staining with isotype-specific conjugates(FITC/PE).Results There was a significant decrease of T-lymphocytes(SWC1+SWC3-)from 90.25% to 44.50% and an increase of the SWC ̄/SWC3 ̄population from 3.56% to 46.13%.The mean percentages of CD1 ̄CD5 ̄,CD2 ̄CD5 ̄and CD4 ̄CD8 ̄thymocytes were 43.63%,60.20% and 67.38% after FK506 treatment,respectively,while 6.01%,2.02% and 28.78% in the controls.Besides the decrease of CD1+CD5+ and CD2+CD5+subsets,a significant decrease of CD4+CD8+phenotypes could be detected in the FK506 group.Conclusion FK506 treatment might lead to inhibition of maturation and differentiation of porcine thymic pregenitots from CD4 ̄CD8 ̄(double-negative)to CD4+CD8+(double positive)in thymus.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Organ Transplantation
Ano de publicação:
2000
Tipo de documento:
Article