Effect of polygona-polysaccharose on learning and memorizing ability and its possible mechanism in Alzheimer disease mice / 中华老年医学杂志

ABSTRACT

Objective To investigate the effect of polygona-polysaccharose on experimental Alzheimer Disease(AD)mice and its possible mechanism. Methods Twenty-six amyloid precursor protein(APP)transgenic mice were randomly divided into high dosage group(HDG),low dosage group(LDG)and model group(MG)which consisted of 9,9 and 8 mice respectively.1 ml of 16%,4% polygona-polysaccharose solution and 1 ml of drinkable tap water were infused into the mouse stomach in HDG,LDG and MG respectively once a day for 45 days.Morris water maze was used to test the mice's proactive learning and memorizing ability.The morphology of cerebral hippocampus was observed by microscope.The content of amyloid-β-protein(Aβ)and the activity of choline acetyltransferase(ChAT)in the cerebral hippocampus were examined by immunochemical staining method. Results Comparisons among treatment groups(including HDG and LDG)and MG showed(1)Escape delitescence was shortened[at seventh day(26.0±9.4)s,(31.2±8.7)s and (39.3±10.9)s,P＜0.05];(2)The frequency of finding the hidden platform within 120 seconds was inereased(5.28±0.76)times,(3.00±0.77)times and(1.00±0.63)times,(P＜0.01);(3)The duration of swimming in objective quadrant(the forth quadrant)within 120 seconds was prolonged [(75.50±8.39)s,(51.39±11.9)s and(36.87±1.25)s,(P＜0.05)].HDG provided better results than those in LDG(all P＜0.05);(4)The activity of ChAT was enhanced and Aβ concentration was decreased for which results mice in HDG showed better than in LDG(all P＜0.05);(5)Morphological study in MG showed the sign of neuron apoptosis such as the reduced number of neuron.the shrinking neuron nuclei,the nucleic membrane being irregular and slightly thickened.However.the neuron in treatment groups were more in number,less transformed and more regularly distrIbuted. Conclusions Polygona-polysaccharose can reduce the accumulation of Aβ in the cerebral hippocampus of AD mice and enhance the activity of ChAT.It can significantly improve the learning and memorizing ability of AD mice and thus proves to be an effective experimental drug for treating AD.