Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
; (53): 1843-1846, 2010.
Article
em Zh
| WPRIM
| ID: wpr-402544
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:Human amniotic epithelial cells(AECs)are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair.Currently,marking and tracing of human AECs remains rarely reported.OBJECTIVE:To explore the efficiency of adeno-associated virus(AAV)vector-medicated green fluorescent protein(GFP)on in vitro cultured human AECs transfection.METHODS:Human amnion samples were harvested and trypsinized to isolate human AECs.The AECs were transfected with AAV-GFP,and the transfection efficiency was detected.RESULTS AND CONCLUSION:Human AECs were successfully primary cultured and passaged in vitro.AAV-GFP-transfected AECs stably and highly expressed GFP,with a transfection efficiency of 58%.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2010
Tipo de documento:
Article