Observation of Reactive Oxygen Species on the Apoptosis of H_2O_2 Cochlear Hair Cell of Organ of Corti in Rat / 听力学及言语疾病杂志

ABSTRACT

Objective To observe the action of H_2O_2 on the hair cells in the organ of Corti of rats.Methods The culture technology of Corti in neonatal rat in vitro was used to establish the model of studying the injury of inner and outer hair cells caused by exogenous H_2O_2. 24 neonatal SD rats of 1～ 5 days were selected and divided into 4 groups with 6 in each group.There were 12 ears in each group①serum-free medium;②0.05 mmol/L H_2O_2;③0.1 mmol/L H_2O_2;④0.5 mmol/L H_2O_2.Forty-eight hours after the tissues cultivation, the tissue was stained by AO and PI.The ratio of apoptotic cells in different groups was studied.Results Apoptosis cells were detected in groups of different concentration of H_2O_2.Outer hair cell were the main target attacked by H_2O_2.But not at apoptotic Sertoli cells.The loss of hair cells on the bottom,middle and parietal turn of basilar membranes varied as the injury to the bottom turn was more severe.The results suggest OFR could cause apoptosis of hair cells,and cochlea damage was dose-dependent in this way.Conclusion Under the conditions of this experiment,exogenous H_2O_2 can cause the apoptosis of hair cells of the organ of Corti in rats,but not related to apoptotic Sertoli cells.