Repair of beagle canine defects with fascia-encapsulated bone marrow mesenchymal stem cells/poly-lactone complex / 中国组织工程研究

ABSTRACT

BACKGROUND: Bone tissue engineering materials/call complex has been able to live in the muscle, subcutanecus tissue, ano other heterotopic bones, or in small mammals to repair bone defect. However, there is still much practical and clinical gap, such as bone tissue engineering and technical ability to repair large bone defects in big mammals, as well as how to promote the in vivo tissue-angineerad bone revascularization process.OBJECTIVE: To observe the bone formation using beagle deep fascia pedicled flap and tissue-engineered bone.METHODS: Beagle bone marrow mesenchymal stem calls were isolated, cultured, and inoculated on poly-lactone (PCL).Bone/bone membrane defect was induced in middle tibia on the left side of beagle. Then, the defect was implanted with fascia-encapsulated bone marrow mesenchymal stem cells (BMSCs), considering as experimental group. The second defect was induced in the middle tibia on the right side of beagle and implanted with BMSCs/PCL, considering as control group. The third defect was induced in 2 additional beagles without any implantation, considering as blank control group. Gross observation, X-ray test, histology, and magnetic resonance perfusion imaging were performed on the models to observe growth and ostecblasts andvasculadzation. RESULTS AND CONCLUSION: There was no new bone formation and blood vessels growth in the blank control group, and the defect was filled by fiber scar tissues finally. After 8-16 weeks, the bone defect was gradually filled by bony tissue, and more calluses which grew in implants were observed. The broken ends of fractured bone were not intact, and pulp cavity was sclerotic.Bone formation in the experimental group was rapid than in the control group. After 6 weeks, a great quantity of calluses was observed; after 8 weeks, stant materials were completely degraded; after 12 weeks, bone defect was succassfully repaired. A greet quantity of cancallated bones was observed, the newborn cavitas medullaris was smooth, and cortical bone was successive and stable. The amount, pore diameter, and distribution of formed blood vessels in the experimental group were superior to those in the control group, suggesting that tissue-engineered bone was able to effectively and rapidly repair bone defect in some animal.Fascia flap could promote the revascularization in vivo of tissue-engineered bone.