Establishment of a stenogamous colony of Anopheles minimus theobald, 1901 in the laboratory

ABSTRACT

An. minimus was collected from Hoa Son village, Luong Son district, Hoa Binh province in November 1997 and maintained in the laboratory of NIMPE by artificial mating techniques. Since 51st generation, a free mating colony in the small cages (with dimensions of 30 x 30 x 30cm) has been established by releasing 200 male and 200 female mosquitoes per cage. The releasing of additional 50 males of An. minimus to the cage has been found to stimulate the mating process in small cage