Preparation of recombinant human zona pellucida 3 protein and its immunologic activity / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1119-1121, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-403564
ABSTRACT
Objective:
To prepare and purify recombinant human zona pellucida 3 protein,and to study its immunologic activity.Methods:
The E.coli BL21 containing recombinant plasmid pGEX4T-1/ huZP3 was induced to express GST-fusion protein by IPTG.After a series of purification procedure,the purified protein was analyzed by SDS-PAGE.After the mice immunized with rhuZP3,the antibody responses against rhuZP3 were detected by ELISA.Results:
The soluble fusion protein was expressed,and purity of rhuZP3 was 95%.Moreover, purity of rhuZP3 could be recognized by anti-human ZP3 in ELISA.Conclusion:
The rhuZP3 is obtained through the preparation of prokaryotic expression system and anti-rhuZP3 antibody has immunological activity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2009
Tipo de documento:
Artigo
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