Production of discrepant monoclonal antibody against HL60 and HL60/ADR by SI technique / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 160-163, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-403903
ABSTRACT
Objective:
To prepare and characterize specific and discrepant mouse hybridoma antibodies on membrane of HL60 and HL60/ADR cell lines.Methods:
BALB/c mice were immunized by subtractive immunization induced Cp(Cyclophosphamide).McAbs were prepared by hybridoma technique,screened and detected by FACS and LSCM.Results:
51 candidates and discrepant antibodies were found,and one of them (5F6) was purified and identified.Conclusion:
Combination of SI with discrepant screening method should facilitate the preparing and identifying discrepant McAbs for identifying antibodies that can distinguish the differences in proteins expressed in HL60 and HL60/ADR,which is a significative and potential method in the research and target therapy associated drug-resistance.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2010
Tipo de documento:
Artigo
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