Detection of receptors of Streptococcus mutants in human whole salivary components / 西安交通大学学报(医学版)

ABSTRACT

Objective To select the acceptor ingredients of Streptococcus mutans protein in the whole saliva through the interaction between adhesion and saliva protein of Streptococcus mutans. Methods After activation and liquid culture of Streptococcus mutans strains of international standards, we took Su-bonding from liquid culture of bacteria secretion in the process of liquid culture by using chilled ethanol extraction. Then su-bonded components underwent electrophoresis analysis, content determination and antigen detection. We collected 15 with high carries and 15 with no carries. Two hours after eating, the whole non-irritating saliva was collected, electrophoresis samples were prepared after freeze-drying, and then stains were separated with SDSPAGE. Western blot detection was used for adhesive protein and Streptococcus mutans' antibody composition. Results The ingredients of Su-bonding secreted on the surface of Streptococcus mutans were obtained in the cultured saliva. There were at least six kinds of components in the extraction of Western blot detection, not limited to the three known kinds of proline-rich protein. Conclusion The adhesion of Streptococcus mutants can be bound with the proteins of saliva. It may be the material basis in Streptococcus mutants adhered to tooth surface.