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The role of DC in anti-CD45RB antibody-induced immune tolerance / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 113-116, 2010.
Artigo em Chinês | WPRIM | ID: wpr-404050
ABSTRACT

Objective:

To investigate the role of dendritic cells (DCs) in the anti-CD45RB antibody-induced immune tolerance,and the mechanism of immune tolerance induced by anti-CD45RB antibodies.

Methods:

DCs were induced by conventional methods(rmGM-CSF,IL-4 and LPS),different doses of anti-CD45RB antibodies were added in the induction process.Then,mature cells were harvested,and flow cytometry(FCM) was used to determine cell phenotypes,cycle and phagocytosis,ELISA method was performed to examine the concentration of IL-12 and the ability of T cell proliferation was observed by mixed lymphocyte culture.

Results:

The DCs preparation was shown a high expression of CD11C and CD83,a low expression of CD86,with strong proliferative and phagocytic capacity after treated with anti-CD45RB antibody.Its secretion of IL-12 was decreased,and it could less effectively stimulate T cell proliferation after treated with anti-CD45RB antibody.

Conclusion:

One of the mechanisms of anti-CD45RB monoclonal antibody-induced tolerance is to generate tolerogenic dendritic cells(tDCs),significantly inhibiting the proliferation of T cells.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2010 Tipo de documento: Artigo