Synthesis and Identification of Hapten and Complete Antigen of Norketa mine / 分析化学
Chinese Journal of Analytical Chemistry
;
(12): 109-112, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-404290
ABSTRACT
Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hap ten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ioni zation time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 111.The antibody with high titer(5.12 × 10~4) was produced after immuning to rabbits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Analytical Chemistry
Ano de publicação:
2010
Tipo de documento:
Artigo
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