Simultaneous Determination of 5-Hydroxymethylfurfural and Nine Kinds of Phenolic Compounds in Rice Wine Using High Performance Liquid Chromatography / 分析化学

ABSTRACT

An analytical method of reversed phase high performance liquid chromatography (RP-HPLC) was developed for simultaneous determination of 5-hydroxymethylfurfural and nine phenolic compounds (including (+)-catechin, (-)-epicatechin, chlorogenic acid, rutin, caffeic acid, protocatechuic acid, syringic acid, ferulic acid and p-coumaric acid) in 20 min.Ten components were detected and separated successfully by Diamonsil C_(18) column (150 mm x4.6 mm, 5 μm) at wavelength of 280 nm and column temperature of 42 ℃, with acetonitrile and 3% acetic acid solution as the mobile phase in gradient elution.The resultant correlation coefficients of the ten compounds were between 0.9911 and 0.9995 with detection limit from 0.2 to 0.5 mg/L, the RSD less than 2.4% and the average recoveries from 89.4% to 98.3%.These experimental results demonstrate that 5-hydroxymethylfurfural and the nine phenolic compounds in different rice wine samples can be determined with the new method for practical uses.