An Expedient Reliable Double Fluorescent Reporter System for φC31 Integrase Function Evaluation / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
;
(12): 929-933, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-404819
ABSTRACT
A reporter system for φC31 integrase was developed in NIH3T3 cells. The reporter plasmid coding green fluorescent protein (GFP) coupled with red fluorescent protein (RFP) was eo-transfected with the plasmid coding φC31 integrase, to show the activity of integrase in the cells. Fluorescence activated cell sorter (FACS) was used to measure the proportion of the cells containing red and green fluorescence. The increment of green cells was positively related to the increase in the transfection with plasmid coding φC31 integrase. Approximately 90% of green cells were observed under a ratio of [plasmid-φC31-integrase]/[reporter plasmid] at 10 1. This suggests that the φC31 integrase reporter system provides a probe for the function of φC31 integrase in cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Progress in Biochemistry and Biophysics
Ano de publicação:
2009
Tipo de documento:
Artigo
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