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The expression of the pancreatic duodenal homeobox-1(PDX-1) in pancreas of experimental type 2 diabetic rat and the effect of rosiglitazone on its expression / 中国糖尿病杂志
Chinese Journal of Diabetes ; (12): 868-870, 2009.
Artigo em Chinês | WPRIM | ID: wpr-405558
ABSTRACT
Objective To observe PDX-1 expression in the pancreas of type 2 diabetic rat model and effects of rosiglitazone (RGZ) on it. Methods Type 2 diabetic rat model was induced by long-term feeding with high-fat foods followed by a single intraperitoneal injection of low dose of streptozotocin (STZ). The morphological changes of pancreas were examined by microscopy and immunohistochemistry. The mRNA levels of PDX-1 and insulin were determined by RT-PCR. PDX-1 protein expression was detected with Western blot. Results The percentage of insulin-positive cells(12.75±2.18),the average optical densities of PDX-1-positive area(0.240±0.051),PDX-1 mRNA level (0.153±0.071)and PDX-1 protein level(0.253±0.028) were significantly lower in untreated diabetic rats than in the controls(42.61±2.68,0.648±0.087,0.49±0.032,0.720±0.036 respectively)(all P<0.01),and in RGZ-treated versus untreated group those parameters increased significantly (all P<0.05~0.01). Conclusions Rosiglitazone could protect islet cell function,and improve PDX-1 expressions in both mRNA and protein levels.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Diabetes Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Diabetes Ano de publicação: 2009 Tipo de documento: Artigo