Screening and analysis of metallo-β-lactamase -producting Pseudomonas aeruginosa in the burn ward / 国际检验医学杂志

ABSTRACT

Objective To compare the resistance of metallo-β-lactamase -producting Pseudomonas aeruginosa between burn ward and other wards, so as to provide basis for reasonable use of antimicrobial agents. Methods BD Phoenix-100 bacteria identification and drug sensitivity analyzer was used to detect Pseudomonas aeruginosa and its susceptibility, and disc synergy method was used for the screening of metallo-v-lactamase -producting Pseudomonas aeruginosa. Results Of 68 strains of Pseudomonas aeruginosa, 9 strains (13.2%) were metallo-β-lactamase (MBL) positive. Among the 9metallo-β-lactamase positive strains, 7 strains were from the burn ward and 2 strains from other wards. The resistance rate of the MBL-producing strains to ceftazidime, cefotaxime, imipenem, ciprofloxacin, amikacin, gentamicin, piperacillin and aztreonam was respectively 100.0%, 100. 0%, 88.9%, 66.7%, 88.9%, 100,0%, 100.0%, and 88.9%, obviously higher than that of non-MBL-pro-ducing Pseudomonas aeruginosa. Conclusion The rate of MBL-producing Pseudomonas aeruginosa is obviously higher in burn ward than that in other wards. The resistance of MBL-producing Pseudomonas aeruginosa is high against commonly used antimicrobial agents. Clinical use of antibiotics should he based on the antimicrobial susceptibility test results.