Identification of the Kiwifruit Germplasms in Jiangxi Province by AFLP and Its Classification Significance / 中国生物化学与分子生物学报

ABSTRACT

The kiwifruit germplasms in Jiangxi province were identified by amplified fragment length polymorphism(AFLP)markers．Four primer pairs that had been selected from 64 ones had detected a total of 190 bands among 31 germplasms of kiwifruit,one hundred and seventy nine bands that representing 94．2％of total bands were polymorphic．The identification rates of 31 germplasms were up to 100％．The result suggests that applying AFLP markers to analyze the kiwifruit germplasms is feasible．Then Clustering analysis the AFLP resuit by UPGMA．The dendrogram indicated that the Dice similarity coefficients of 31 germplasms of kiwifruit ranged from 0．50 to 0．85,it suggested that their genetic relationships weren't near．Thirty one germplasms could divide into four groups according to the Dice similarity coefficient 0．56．Sect．Leiocarpae and Sect．Maculatae clustered into one group；A．Melliana of Sect．Strigosae was one group；A．Chinensis var. Rufopulpa,A．Eriantha,A．Fulvicoma var．Lenata,A．Styracifolia and A．Jiangxiensis in Sect．Stellatae clustered into the same group；but the two germplasms of Sect．Stellatae,A．Deliciosa and A．Chinensis clustered into the other group．Interestingly,the‘Ganmi-5’was in the same rank with A．Chinensis from the dendrogram,but it was the variety of A．Chinensis according to the traditional classification．It is necessary that the‘Ganmi-5’should be further researched in classification．The genetic relationships among the kiwifruit germplasms in Jiangxi province were identified and characterized by themolecular method,which was consistent with the traditional classification in certain degree and provided new evidences for the classification of the kiwifruit germplasms．