Area of atherosclerotic plaque in mice with apolipoprotein E genetic defect and serum level of anti-oxidized low density lipoprotein antibody

Zhihong TANG; Min XIA; Huilian ZHU; Jing MA; Wenhua LING

ABSTRACT

BACKGROUND:

The oxidation of low density lipoprotein (LDL) is a key influencing factor in the occurrence and development process of atherosclerosis. How is the merit of the method for the detection of the level of anti-serum oxidized LDL (ox-LDL) antibody on the evaluation of atherosclerotic plaque?

OBJECTIVE:

To study the method for the detection of serum anti-ox-LDL antibody in mice with apolipoprotein E (Apo-E) genetic defect to analyze the merits of serous level of anti-ox-LDL antibody on the evaluation of the area of atherosclerotic plaque in mice with Apo-E genetic defect.

DESIGN:

Single factor analysis of variance (a case-controlled study)

SETTING:

Laboratory of nutrition and metabolism diseases in a university.

PARTICIPANTS:

Mice with Apo-E genetic defect were grouped into positive group (series C57B L/6J, n = 15), while normal mice were grouped into control group (series C57BL/6J, n = 15).

INTERVENTIONS:

Mice of two groups were fed in separate cage on laminar flow shelf for free drinking and eating. The venous blood was drawn from the orbit of mice after 16 weeks for the separation of mice serum. The level of anti-ox-LDL antibody was detected by enzyme-linked immunosorbent assay (ELISA) in the separated serum from either mice with Apo-E genetic defect or normal mice. The area of atherosclerotic plaque was measured by image analysis after oil red O staining.MAIN OUTCOME

MEASURES:

ox-LDL level and atherosclerotic plaque area in mice with Apo-E genetic defect or normal mice.

RESULTS:

Anti-ox-LDL antibody level of mice with Apo-E genetic defect was[ (0. 079 ±0. 028)% ], which was significantly higher than [(0. 012± 0.001 )% ] of normal mice ( F= 10. 666, P ＜ 0.01 ). The area of atherosclerotic plaque of mice with Apo-E genetic defect was (26. 25 ± 9.20) %, which was also significantly higher than 0% of normal mice, and moreover, there was a significant correlation between these two factors ( r =0. 638, P ＜ 0.01).

CONCLUSION:

Serum level of anti-ox-LDL antibody in mice with Apo-E genetic defect is closely correlated with the area of atherosclerotic plaque,which is an important indicator for the generation of atherosclerosis in mice with Apo-E genetic defect.