Polymerase Chain Reaction Detection for Porcine Endogenous Retrovirus in 4 Pig Cell Lines / 中国兽医学报
Chinese Journal of Veterinary Science
; (12): 1-3, 2003.
Article
em Zh
| WPRIM
| ID: wpr-410131
Biblioteca responsável:
WPRO
ABSTRACT
Porcine endogenous retroviruses (PERV) can infect human cell in vitro, which raised widely concerns re-garding the transmission of PERV to xenograft recipients. It's essential to establish a method for detection of PERV.3 pairs of primers were synthesized according to the sequence of gag, pol and env gene of PERV. Polymerase chainreaction (PCR) and reverse transcription PCR (RT-PCR) assays were performed for detection of PERV provirusDNA and PERV specific mRNA. The results showed that provirus DNA and mRNA of PERV existed and expressedin all 4 tested cell lines. The sizes of amplified fragments are identical with the predicted. These methods may be suit-able for monitoring PERV in other cells or tissue.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Veterinary Science
Ano de publicação:
2003
Tipo de documento:
Article