Determination of Fluorouracil in Human Serum With HPLC / 中国药房
China Pharmacy
;
(12): 227-228, 2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-410699
ABSTRACT
OBJECTIVE:
To determine the content of 5-Fu in human serum.METHODS:
Serum protein was precipitated with trichloroacetic acid .HPLC was used for determination of 5-Fu .The column was Shimpack CLC C18(150 × 6mm, 5μm)with YWG for protection.The mobile phase was 0.25% KH2PO4-acetonitrile(98 2, pH7.0) .The flow rate was 1ml/min.The detection wavelength was 265nm.Two standard curves were made in the concentrations of 0.195~6.250μg/ ml and 6.25~200.00μg/ml, respectively.RESULTS:
The calibration curves revealed linearity in the range of 0.195~200μg/ml.The with-in-day recoveries and RSDs of 5-Fu at 0.8, 4, 20 and 100μg/ ml concentration points were 104. 88%, 1.95%; 104.58%,1.38%; 101.40%, 0.39%and 99.14%, 0.37%, and the between-day recoveries and RSDs of 5-Fu were 105.12%, 2.02%;106.30 %, 0.78 %; 100.60 %, 0.65 % and 99.38 %, 0.92 % respectively.CONCLUSION:
This method is rapid, accurate and suitable for pharmacokinetical study and conventional monitoring of 5-Fu.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS