Determination of Serum Concentrations of Propofol by RP－ HPLC / 中国药房

ABSTRACT

OBJECTIVE： A RP－ HPLC method was established for determination of serum concentration of propofol in man METHODS： The mobile phase consisted of methanol－ water（ 68∶ 32v/v） The detection was carried out at wave length 258nm and flow rate 1ml/min with carbamazepine as internal standard RESULTS： The retention times of propofol and carbamazepine were 9 20 and 5 16 min， respectively The mean recovery of propofol was 99％  The within－ day and inter－ day variations were all less than 10％  Propofol and carbamazepine were seperated well The assay linearity was obtained in the range of 1～ 16μ g/ml in serum（ r=0 9 994）  CONCLUSION： The method is sensitive， simple and reliable for the determination of propofol concentration