Effect of urotensin Ⅱ on nitric oxide synthese/nitric oxide pathway in rat aorta / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6): 147-149, 2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-411041
ABSTRACT
Objective:
To study the effect of urotensin(U Ⅱ) on nitric oxide synthase(NOS)/nitric oxide(NO) system in rat aorta.Methods:
The aortic slices were incubated in vitro. The medium nitrite content, vascular NOS activities and cGMP content were measured.Results:
After incubation of aorta for 1.5-3.0 h, UⅡ(10-9-10-7 mol*L-1) increased nitrite production ( 14.8%-80.9%, P<0.01) in dose-dependent manner. U Ⅱ significantly stimulated the total NOS activities, mainly activated constitutive NOS (P<0.05 or P<0.01), however, the inducible NOS activities were not altered (P>0.05). Incubation of aortic slices with U Ⅱ (10-9-10-7 mol*L-1) increased vascular cGMP content in dose-dependent manner. Co-incubation vascular tissue with U Ⅱ and GN-L-nitro-arginine, an inhibitor of NOS, obviously inhibited the U Ⅱ-induced NOS activation, NO production and cGMP formation.Conclusion:
The results suggest that U Ⅱ can activate the vascular NOS/NO pathway, increasing tissue NO production and cGMP content.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Peking University(Health Sciences)
Ano de publicação:
2001
Tipo de documento:
Artigo
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