Effect of quercetin on cell cycle and nitroxide in hydrogenperoxide-induced of endothelial cells / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 211-213, 2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-411552
ABSTRACT
AIM To study the effects of quercetin (Que) on cell cycle and nitric oxide in H2O2-induced the human umbilical vein endothelial cell line(ECV-304). METHODS The experiment were performed in culture of H2O2-induced human umbilical vein endothelial cell line(ECV-304) in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Nitroxide(NO) of ECV-304 was monitored as NO2- with colorimetry. RESULTS H2O2 inhabited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24 h before H2O2 exposure significantly increased the cell viability and S-phase and G2M-phase cells. Que reduced lactate dehydrogenase(LDH) and increased the level of nitric oxide in H2O2-induced ECV-304. CONCLUSION These results demonstrate that Que can produce the protective action on H2O2-induced cultured ECV-304 and its effect of action may be related to level of nitric oxide.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS