A FAST DETERMINATION OF POLYUNSATURATED FATTY ACIDS PRODUCED BY MARINE FUNGUS* / 微生物学通报

ABSTRACT

A fungus that is capable of producing EPA and DHA was isolated from seawater. A fast extraction and methyl esterification of microbial lipid were established as well as their content determination by capillary gas chromatography using external calibration method. The results showed that the methyl ester prepared directly form the wet mycelia treated with CHCl3-KOH-CH3OH is less time-consuming and the method fits for the fast determination. To determine its EPA and DHA content,the wet mycelia was hydrolyzed according to Weibul 's method and the lipid obtained was methyl esteri.fied with KOH-MeOH,the results obtained by capillary gas chromatography using external calibration method were 7. 32% and 7. 58% respectively.