Study of the Regulatory Effects of Estrogen Progesterone and Gona dotropins on Growth of Ovarian Cancer Cell Line H0-8910 in Vitro / 中山大学学报(医学科学版)

ABSTRACT

【Objective】 The study was carried out to investigate t he effects of estradiol(E2), follicle-stimulating hormone(FSH) and progestero ne(P) on the proliferation of ovarian cancer cell line H0-8910 in vitro. 【 Method】 Cultivating human ovarian serous cystadenocarcinoma cell line H0-8910 in vitro, comparing cell numbers using MTT method, flow cytometry detecting cell cycle. 【Results】 After incubation with 10-12 mol/L E2 or 10 μg/L FSH for 48 hours, cell proliferation increased significantly. E2 and FSH increased th e cells percentage in Sphase and G2/M phase of cell cycle respectively P could inhibit H0-8910 cells growth significantly in a dose-dependent manner. 【Conc lusion】 It is suggested that E2 and FSH can stimulate the proliferation of ov arian cancer cell, P can inhibit cell growth.