Protective Effects of Epigallocatechin Gallate After UV Irradiation of Cultured Human Lens Epithelial Cells
Korean Journal of Ophthalmology
;
: 183-186, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-41299
ABSTRACT
PURPOSE:
To evaluate the protective effects of epigallocatechin gallate (EGCG) against UV irradiation of cultured human lens epithelial cells.METHODS:
We irradiated cultured human lens epithelial cells with a 30-second pulse from a UV lamp with an irradiance of 0.6 mW/cm2. Five minutes and 1 hour after UV irradiation, we administered 0, 5, 10, 15, 25, 50, or 100 uM EGCG. The cell number was measured with a microscopic counting chamber and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.RESULTS:
Compared to untreated cells, the total number of cultured human lens epithelial cells was markedly higher after UV irradiation. In a dose-dependent manner, viability was also higher in EGCG-treated cells.CONCLUSIONS:
EGCG increased the cell count and cell viability after UV irradiation of cultured human lens epithelial cells, indicating that EGCG can protect lens epithelium against UV damage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Lesões por Radiação
/
Protetores contra Radiação
/
Sais de Tetrazólio
/
Tiazóis
/
Raios Ultravioleta
/
Catequina
/
Contagem de Células
/
Sobrevivência Celular
/
Células Cultivadas
/
Relação Dose-Resposta a Droga
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2008
Tipo de documento:
Artigo
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