Effect of CD44 antibody on hyaluronic acid degradation caused by retinal Müller cell / 中华眼底病杂志

ABSTRACT

Objective To observe the effect of CD44 antibody on the hyaluronic acid (HA)generation) were cultured in three different medium without HA (group 1),0. 01 mg/ml HA (group 2),10 μg/ml HA and CD44 antibody (group 3). The cells in the group 2 and 3 were pre-cultured with HA and CD44 antibody, and the supernatant was collected. HA-substrate gel electrophoresis was performed for HA degradation, while ELISA-like method was performed for HA-binding protein. Results HA-substrate gel electrophoresis showed white light double-band on blue background in groups 1 and 3, thicker double-band or bright de-colored blocks in group 2. ELISA-like method showed that the absorbance (A) value of groups 1,2 and 3 were 0.310 ± 0.025, 0.093 ± 0.051, 0.025 ± 0.069 respectively. The A value of group 2 was obviously lower than that of group 1 (t=28.1, P＜0.01); the A value of group 3 was significantly higher than that of group 2 (t=26.9, P＜0.01), but was the same as group 1 (t=4.92, P＞0.05). Conclusion CD44 antibody can inhibit the interaction between Miller cells and HA, and thus reduce the HA degradation.