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The screening of liver cancer specific binding peptide from serum / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery ; (12): 253-256, 2011.
Artigo em Chinês | WPRIM | ID: wpr-413953
ABSTRACT
Objective To find the liver cancer specific peptide for serological screen of liver cancer patients via screening phage-display peptide library. Methods Fifteen sera from liver cancer patients and physical examinates were collected for the four-round screening with Ph. D. 12TM phage display peptide kit. Highly specific phage monoclones were selected based on the ELISA results of the serological assay. The peptide labeled with FITC was synthesized according to the DNA sequencing of the optimal monoclone and tested with serum via fluorescent imagery. Results Nine highly specific monoclones were found among 50 selected ones after 4 rounds of screenings. The positive rate of the optimal monoclone,ZH-3, reached 46.7 %. The peptide sequence of ZH-3 was concluded by DNA sequencing as SAHGTSTGVPWP. Desirable specificity and affinity were also shown in the serum of liver cancer patients. Conclusion The peptide ZH-3 can be used as a diagnostic reagent for liver cancer.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Chinês Revista: Chinese Journal of Hepatobiliary Surgery Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Chinês Revista: Chinese Journal of Hepatobiliary Surgery Ano de publicação: 2011 Tipo de documento: Artigo