Effect of sympathetic activity on peripheral immune function of middle cerebral artery occlusion in rats / 国际脑血管病杂志

ABSTRACT

Objective To investigate the relationship between the changes of the sympathetic nervous system activity and the peripheral cellular immunodepression after cerebral infarction.Methods An adult male Sprague-Dawley rat model of middle cerebral artery occlusion (MCAO)was induced.A double-antibody sandwich enzyme-linked immunosorbent assay was used to detect the levels of serum proinflammatory cytokines interleukin(IL)-1β,interferon-γ (INF-γ)and anti-inflamatory cytokines IL-10.A competitive enzyme-linked immunosorbent assay was used to detect the levels of serum metanephrine(MN)and normetanephrine(NMN).After blocking sympathetic activity by injecting β -blocker propranolol intraperitoneally,the sympathetic activity and the levels of pro/anti-inflammatory cytokines were detected.Results The levels of serum MN and NMN at 6 hours after MCAO began to increase in the cerebral infarction group compared with the sham operation group.They reached the peak at 72 hours,and then decreased slowly.However,they were still higher than those in the sham operation group 2 weeks after MCAO(P ＜ 0.01 ).At 72 hours after MCAO,the levels of MN and NMN in the propranolol intervention group were decreased significantly(P ＜0.01 ),the levels of proinflammatory cytokines IL-1β and INF-γwere increased significantly(P ＜0.01 ),and the levels of anti-inflammatory cytokine IL-10 were decreased significantly(P ＜0.01).Conclusions When sympathetic nervous system is overactivated after cerebral infarction in rats,the peripheral cellular immunity is in a state of depression;the peripheral immunodepression will be fundamentally reversed after blocking the sympathetic function,and this suggests that the overactivation of sympathetic nervous system may play a important role in the process of immunodepression after cerebral infarction.