Expression and cytotoxicity of a human interleukin-6 tumor necrosis factor derivative fusion protein (5'IL6-TNF△) in vitro / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 482-483, 1999.
Artigo
em Chinês
| WPRIM
| ID: wpr-414772
ABSTRACT
Objective:
To develop an agent that is more active against receptor-bearing target cells without increasing the toxic effect on non-target cells.Methods:
By the use of molecular biology techniques,we designed and constructed a fusion protein 5'IL6-TNF△ by connecting the human interleukin-6 (hIL-6) gene and a human tumor necrosis factor α derivative (TNF△) gene througha synthetic linker sequence followed by subsequent expression in E. Coli.Results:
In cytotoxicity assay with myeloma cell line U266, the normal type of 5' IL6-TNF△ showed an antitumor activity 3 times higher than that of TNF△;and the antitumor activity of 5'IL6-TNF△ blocked by IL-6Rwas only 1/30 of that of normal type of 5' IL6-TNF△. Meanwhile,the 5'IL6-TNF△ blocked by an ti-TNF antibody did not show any cytotoxicity to U266 cells. In activity assay with L929 cells ,the toxic effect of the fusion protein was found 1/22 of that of TNF△.Conclusion:
The 5'IL6-TNF△fusion protein might be a useful cytotoxic agent in cancer treatment.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Jilin University(Medicine Edition)
Ano de publicação:
1999
Tipo de documento:
Artigo
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