Effects of extract of termitarium on the climbing ability and life span of Parkinson's disease Drosophila / 中华老年医学杂志

ABSTRACT

Objective To observe the eftccts of the extract of termitarium on the climbing ability and life span of Parkinson disease (PD)drosophila(Ddc GAL4/+ ;UAS-wild type α-synuclein/I ),and to explore its mechanism of anti- PD. Methods The termitarium was extracted by ethanol.PD drosophilas overexpressed α-synuclein were fed with 1,10 and 100 mg of termitarium per 100 g culture medium.The climbing ability and life span of drosophilas and pathological changes of its brain tissue (Congo red staining) were observed and compared among different concentrations. Results Termitarium enhanced the climbing ability of PD drosophilas and the most significant improvement appeared from 18 d to 36 d after eclosion,increasing with more termitarium concentrations and growing age of PD drosophilas.Termitarium also prolonged the life span among which male and female drosophila were longer 31.82 ％ ～ 3.34 ％,1.47 ％ ～ 3.34 ％ as compared with PD drosophila control (all P＜0.05).The brain cells number in the PD drosophilas were increased and empty bubble was decreased by rermitarium, accompanying with improvement of behavior and life span.Conclusions Termitarium can improve behavior and prolong life span of PD drosophilas.