Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes
Immune Network
;
: 42-49, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-41912
ABSTRACT
BACKGROUND:
In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-alpha and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured.METHODS:
To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-alpha and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity.RESULTS:
The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-alpha and IL-8 production in HaCaT cells and a significant inhibition of TNF-alpha was shown when cells were treated with 500 and 1,000 microg/ml of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than 60 microg/ml of KRG extracts were significantly different in human dermal fibroblast cells.CONCLUSION:
Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Picratos
/
Superóxido Dismutase
/
Compostos de Bifenilo
/
Ensaio de Imunoadsorção Enzimática
/
Queratinócitos
/
Interleucina-8
/
Fator de Necrose Tumoral alfa
/
Dermatite Atópica
/
Fibroblastos
/
Panax
Limite:
Humanos
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2011
Tipo de documento:
Artigo
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