Effect of liver depression on Th17 and treg in asthma ratsi / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 989-993, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-420487
ABSTRACT
Objective To investigate the effect of liver depression (Liver Qi Stagnation) on Th17,Treg,IL-17,IL-10 and airway inflammation in asthmatic rats,and to clarify the immune mechanism of asthma with liver depression.Methods Established the combined with disease and syndrome model of asthma with liver depression.Collected the bronchoalveolar lavage fluid (BALF) to count the total and differential cell.Lung tissue was observed in microscope; the proportion of Th17 cells and Treg cells of CD4 +T cells in peripheral blood was measured by flow cytometry; the levels of IL-17 and IL-10 were determined by ELISA.Results The total number of inflammatory cells[(96.86±4.43)× 107/L,(88.22±3.22)× 107/L],the proportion of eosinophils [(27.58 ±4.65) %,(22.67±2.43) %],Th17 cells[(6.86±0.98) %,(6.01 ±0.77) %] and IL-17 level [(48.88± 8.06)pg/ml,(43.24± 6.32) pg/ml] of asthma in liver depression group and asthma group were significantly higher than the control group [(30.58 ± 2.49) × 107/L,(0.78 ± 0.12) %,(2.80± 0.82) %,(24.11 ±3.40)pg/ml]; Treg cells [(3.09±0.55) %,(3.96±0.66) %] and IL-10 level [(19.79±2.80) pg/ml,(20.29±3.12) pg/ml] were significantly lower than the control group [(8.02± 1.26) %,(30.79 ± 4.01) pg/ml].The total number of inflammatory cells (96.86 ±4.43) × 107/L,the proportion of eosinophils (27.58±4.65) % and Th17 cells(6.86±0.98) % and IL-17 level (48.88±8.06)pg/mL of asthma in liver depression group were significantly higher than the asthma group (88.22 ± 3.22) × 107/L,(22.67 ± 2.43) %,(6.01 ± 0.77) %,(43.24 ± 6.32) pg/ml;the proportion of Treg cells (3.09 ±0.55)% was significantly lower than the asthma group (3.96± 0.66)%; and the lung histopathology symptoms was more severe than asthma group.Conclusion Liver Qi Stagnation can promote the inflammation of asthma,the imbalance of Th17/Treg and IL-17 level to aggravate the asthma.Liver depression is one of the major internal factors in recurrent episodes of asthma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
International Journal of Traditional Chinese Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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