An enzymatic method for the detection of human serum albumin
Experimental & Molecular Medicine
;
: 103-105, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-42074
ABSTRACT
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Sefarose
/
Albumina Sérica
/
Química Clínica
/
Cromatografia em Gel
/
Isoenzimas
/
L-Lactato Desidrogenase
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2001
Tipo de documento:
Artigo
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