Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene
Experimental & Molecular Medicine
;
: 76-82, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-42078
ABSTRACT
5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-beta2 gene. We suggest that the transcription of PLC-beta2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfolipases Tipo C
/
Ligação Proteica
/
Transcrição Gênica
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Transfecção
/
Cloranfenicol O-Acetiltransferase
/
Endonucleases Específicas para DNA e RNA de Cadeia Simples
/
Células Cultivadas
/
Mutagênese Sítio-Dirigida
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2001
Tipo de documento:
Artigo
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