Apoptotic Effects of 6-Gingerol in Human Breast Cancer Cells
International Journal of Oral Biology
; : 223-228, 2015.
Article
em En
| WPRIM
| ID: wpr-42179
Biblioteca responsável:
WPRO
ABSTRACT
6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Mama
/
Neoplasias da Mama
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Linhagem Celular
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Apoptose
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Células Epiteliais
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Caspase 3
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Células MCF-7
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Citometria de Fluxo
Limite:
Humans
Idioma:
En
Revista:
International Journal of Oral Biology
Ano de publicação:
2015
Tipo de documento:
Article