Liquid chromatography-mass spectrometry for measurement of serum 25 ( OH ) D level / 中华临床营养杂志

ABSTRACT

Many diseases are associated with vitamin D deficiency.Serum 25 (OH)D levels can serve as objective indicators of the nutritional status of vitamin D.Liquid chromatography-mass spectrometry (LC-MS) method may be applied for routine measurement of 25 ( OH ) D levels in clinical practice.This article reviewed the significance of detecting 25 (OH) D2 and 25 (OH) D3,compared the advantages and disadvantages of different 25 (OH) D measurement methods,and introduced the application of LC-MS method for routine detection of serum 25 (OH) D2 and 25 (OH) D3 levels following the standard by National Institute of Standards and Technology of America.