Effect of Anti - idiotype Antibody on Anti - DNA Antibody Production by Hybridoma Cells / 대한면역학회지
Korean Journal of Immunology
;
: 513-524, 1997.
Artigo
em Coreano
| WPRIM
| ID: wpr-42340
ABSTRACT
Anti-idiotype antibody (anti-id Ab) which recognizes idiotope in the variable region of immunoglobulin (Ig) can regulate Ab production by B cells in vivo and in vitro. Although it has been reported that anti-id Ab can suppress IgM production by lymphocytes or hybridoma cells without suppression of cell proliferation, the regulatory mechanism of anti-id Ab is not completely understood. We studied the effects of anti-id Ab on the production of IgG class anti-DNA Ab by hybridoma cells, on the proliferation of cells, and on the transcription levels of Ig genes. In contrast to suppressive effect of anti-id Ab on the production of IgM previously reported by others, stimulatory effects of anti-id Ab on the production of IgG by hybridoma cells as well as the proliferation of these .cells were observed. However, little effect of anti-id Ab on the transcription levels of Ig genes was observed. These results suggest that anti-id Ab can increase Ab production by stimulation of cell proliferation. Furthermore, these results suggest that the effect of anti-id Ab on the production of Ab may be determined by the difference in class of Ab produced by hybridoma cells following the treatment with anti-id Ab.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Imunoglobulina G
/
Imunoglobulina M
/
Imunoglobulinas
/
Genes de Imunoglobulinas
/
Linfócitos B
/
Linfócitos
/
Proliferação de Células
/
Hibridomas
/
Formação de Anticorpos
Idioma:
Coreano
Revista:
Korean Journal of Immunology
Ano de publicação:
1997
Tipo de documento:
Artigo
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