Role of N-cadherin in cranial neural crest delamination during chick embryogenesis / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 1269-1274, 2012.
Article
em Zh
| WPRIM
| ID: wpr-424007
Biblioteca responsável:
WPRO
ABSTRACT
AIM: To investigate the role of N-cadherin in the delamination of neural crest cells. METHODS: The normal expression of N-cadherin in neural tube was identified using in situ hybridization. The cells with N-cadherin over expression were obtained by transfection of wild-type N-cadherin (wt-N-cadherin) ,and the cells with N-cadherin silencing expression were obtained by transfection of dominant-negative N-cadherin (dn-N-cadherin). The migration of cranial neural crest cells was determined by the technique of immunohistochemistry. RESULTS: Either overexpression or down-regulation of N-cadherin significantly affected the migration of cranial neural crest cells. CONCLUSION: Delamination and migration of the cranial neural crest cells rely on the relative N-cadherin expression in the neural tube during neurulation.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2012
Tipo de documento:
Article